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SBCLNG EFF DH5@ COMP CELLS:Store:-68℃ to -85℃
详细描述
描述
Subcloning Efficiency™ DH5α™ Competent Cells are a versatile strain of chemically competent cells that provide a transformation efficiency of > 1 x 106 cfu/µg plasmid DNA. Subcloning Efficiency™ DH5α™ Competent Cells are an economical solution for routine subcloning procedures or any application where the starting DNA is not limiting.Features of Subcloning Efficiency™ DH5α™ Competent Cells:
• Designed for general, everyday use
• Contain genetic markers useful for general applications
Ideal for routine subcloning procedures
Subcloning Efficiency™ DH5α™ Competent Cells are recommended for routine subcloning of genes into plasmid vectors but are not suitable for the generation of cDNA libraries. These economical cells yield >1 x 106 transformants/µg control DNA per 50 µL reaction.
Flexible cloning capabilities
Subcloning Efficiency™ DH5α™ Competent Cells contain the following genetic markers resulting in these benefits:
• lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
• recA1 ensures increased insert stability and prevents unwanted recombination
• endA1 improves the yield and quality of plasmid DNA prepared from minipreps
• DH5α™ competent cells support replication of M13mp vectors but do not support plaque formation
Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-
规格
| Competent Cell Type: | Chemically Competent |
|---|---|
| Species: | E. coli |
| Format: | Tube(s) |
| Transformation Efficiency: | > 1x10^6 |
| Bacterial or Yeast Strain: | DH5α™ |
| Product Size: | 40 reactions |
| Blue-White Screening: | Yes |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Reduces Recombination: | Yes |
| Cloning Methylated DNA: | No |
| Improves Plasmid Quality: | Yes |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| F' Episome (to make ssDNA): | Lacks F' Episome |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| T1 Phage - Resistant (tonA): | No |
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Shipping Condition: | Dry Ice |
内容及储存
Contains:
• Subcloning Efficiency™ DH5α™ Competent Cells: 4 vials, 500 µl each (total of 2 ml)
• pUC19 DNA (100 pg/ul): 1 vial, 20 µl
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C.
• Subcloning Efficiency™ DH5α™ Competent Cells: 4 vials, 500 µl each (total of 2 ml)
• pUC19 DNA (100 pg/ul): 1 vial, 20 µl
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C.
