CHOventure, Starter Kit, Media System中国仓鼠卵巢细胞扩增培养基套装
详细描述
CHOventure is a chemically defined growth medium for efficient high-yield protein expression/production in batch and fed-batch cultivation systems with CHO cell lines. The CHOventure product line is complemented by two corresponding feeding supplements, CHOventure Feed A and Feed B.CHOventure 培养基是一种化学成分明确的生长培养基，用于在 CHO 细胞系的批式和流加培养系统中进行高效、高产的蛋白质表达/生产。CHOventure 产品线还配有 CHOventure Feed A 和 Feed B 两种相应的补料添加物。
Storage:+2°C to +8°C
Sterility:Sterile-filtered
| Amount | Component | Volume | Cat-No. |
| 1X | CHOventure Growth Medium,中国仓鼠卵巢细胞扩增培养基 with Hypoxanthine, with Thymidine, with Pluronic, w/o Insulin | 500 ml | VEN-500ML |
| 2X | CHOventure Feed A, Feeding Supplement for CHO cells, 中国仓鼠卵巢细胞添加剂A不含胰岛素，不含L-谷氨酰胺w/o Insulin, w/o L-Gl | 100 ml | VENFA-100ML |
| 2X | CHOventure Feed B, Feeding Supplement for CHO cells, 中国仓鼠卵巢细胞添加剂B不含胰岛素，不含 L-谷氨酰胺w/o Insulin, w/o L-G | 10 mL | VENFB-10ML |
产品描述
我们与瑞士 CDMO 公司 ExcellGene（专门提供复杂的蛋白质表达服务）合作，开发了 CHOventure——一个适用于多种中国仓鼠卵巢 (CHO) 细胞系的新型培养系统。
CHOventure 生长培养基专为重组蛋白的高产表达而设计，效价最高可达 15 g/L。CHOventure 具有稳健且可扩展的生长性能，可适用于从小规模实验到大规模生物反应器的各种场景。为了实现稳定高效的表达，CHOventure 采用了化学成分明确的配方，并添加了有价值的微量元素、脂质以及次黄嘌呤/胸苷。
CHOventure 已在多种不同类型的 CHO 细胞（包括 CHO DG44 和 CHO DXB11）上成功进行了生长性能测试。如果细胞先前已在化学成分明确的系统中培养，则可以轻松地逐步适应，甚至直接适应 CHOventure。
CHOventure 产品线包含一种生长培养基和两种补料添加物——CHOventure Feed A 和 B，可为细胞培养补充额外的营养。我们的培养基和补料经过设计和优化，适用于高密度悬浮培养，并能在批式和流加培养系统中实现最大化的产量。模块化的补料系统允许进行组合变化和调整，以实现高效的重组 CHO 细胞培养。
Feed A 含有浓缩氨基酸、维生素、盐类、微量元素和碳源的均衡混合物。
Feed B 由额外的特定氨基酸组成，完善了整个培养基系统。
CHOventure 特点一览
- 最大化细胞活力、生长和生产率
- 通过使用正确的成分，保持一致的产品质量
- 支持多种 CHO 细胞类型
- 完全可扩展的系统
Description
In cooperation with ExcellGene, a Swiss located CDMO, specialized in sophisticated protein expression service, we developed CHOventure, a new cultivation system for a broad range of Chinese Hamster Ovary (CHO) cell lines.
CHOventure growth medium is designed for high-yield expression of recombinant proteins, reaching titers up to 15 g/L. CHOventure features robust and scalable growth performance, from small-scale experiments to large-scale bioreactors. For stable and efficient expression, CHOventure is a chemically defined composition supplemented with valuable trace elements, lipids, and hypoxanthine/thymidine.
CHOventure has been successfully tested for growth performance of various different CHO cell types, including CHO DG44 and CHO DXB11. Adaptation may be easily performed sequentially or even directly, when cultured in a chemically defined system previously.
The CHOventure product line contains a growth medium and two feeding supplements, CHOventure Feed A and B, which sustain cell cultures with additional nutrients. Our medium and feeds were designed and optimized for high-density suspension cultures and maximized production in batch- and fed-batch cultivation systems. The modular feed system allows for combinatorial variations and adaptations for efficient recombinant CHO cultivation.
Feed A contains a balanced mixture of concentrated amino acids, vitamins, salts, trace elements and a carbon source.
Feed B is composed of additional selected amino acids and completes the media system.
CHOventure features at a glance:
- Maximizes viability, cell growth, and productivity
- Balances consistent product quality by using the right ingredients
- Supports a broad range of CHO cell types
- Fully scalable system
