详细描述

DMEM/F-12 是一种广泛应用的基础培养基，可用于支持多种不同类型细胞的生长。由于该配方结合了 DMEM 和 Ham's F-12 的组分，因此提供了丰富的营养混合物。

Cat-No:DMEM-12-HXA
Volume:500 ml
Storage:+2°C to +8°C
Sterility:Sterile-filtered

DMEM/F-12 作为一种基础培养基，广泛应用于细胞培养领域。它为多种哺乳动物细胞系的生长提供基本营养成分，尤其在添加胎牛血清（FBS）后效果更佳。
既往研究表明，DMEM 可成功用于原代细胞（如成纤维细胞、神经元、人脐静脉内皮细胞、平滑肌细胞）以及细胞系（如 HEK-293、HeLa、Cos-7 和 PC-12）的培养。
DMEM/F-12 是 Dulbecco 改良 Eagle 培养基（DMEM）与 Ham's F-12（Ham 营养混合物 F-12）按 1:1 比例混合而成的配方。本款 DMEM/F-12 配方补充有 15 mM HEPES，且不含 L-谷氨酰胺。

添加 HEPES 的 DMEM/F-12 配方通常用于无血清培养应用。在此类情况下，培养基中需补充 15 mM HEPES，以弥补缺失的缓冲能力。
Dulbecco 改良 Eagle 培养基基于 Eagle 最低必需培养基（EMEM）开发，但其氨基酸和维生素浓度提高了四倍。而 Ham's F-12 则基于 Ham's F-10 培养基。
一种非常普遍的做法是在 DMEM 中添加胎牛血清（FBS），通常浓度为 10%。由于培养基中不含有细胞生长所必需的生长激素、脂质或蛋白质，因此这种补充往往是必需的。
DMEM 含有缓冲体系以维持 pH 水平，通常还添加酚红作为 pH 指示剂。在 DMEM 或任何含有碳酸氢盐缓冲体系的培养基中培养细胞时，必须置于 5–10% CO₂ 的受控环境中。若添加了酚红，可通过颜色变化监测 pH 值，范围从 pH 6.2（黄色）至 pH 8.2（红色）。
Capricorn Scientific 生产的所有高品质细胞培养基均依据原始发表配方制造。我们提供为保证培养基性能稳定且一致所必需的改良配方。我们所有经典 DMEM 系列产品均已通过测试，能够为您的细胞生长提供最佳支持。

Description
DMEM/F-12 is widely used in cell culture as a basal medium. It provides basic nutrients for growth of various mammalian cell lines, especially when supplemented with FBS.

In the past, DMEM was shown to successfully cultivate primary cells, such as fibroblasts, neurons, HUVECs, smooth muscle cells, or even cells lines, such as HEK-293, HeLa, Cos-7, and PC-12.

DMEM/F-12 is a 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12 (Ham’s Nutrient Mixture F-12). This formulation of DMEM/F-12 is supplemented with 15 mM HEPES and comes without L-glutamine.

Formulations of DMEM/F-12 with HEPES are usually used for serum-free applications. In these cases, the medium is supplemented with 15 mM HEPES to compensate the missing buffering capactiy.

Dulbecco's Modified Eagle Medium is based on Eagle's Minimal Essential Medium (EMEM) but has a fourfold higher concentration of amino acids and vitamins. Ham's F-12, on the other hand, is based on Ham's F-10 medium.

A very common practice is the supplementation of DMEM with FBS, typically at a concentration of 10%. This is often necessary because the medium does not contain any growth hormones, lipids, or proteins that are essential for cell growth.

DMEM contains a buffer to maintain pH levels and usually comes with phenol red as a pH indicator. Cells cultivated in DMEM, or any other medium containing the bicarbonate buffer system, must be kept in a controlled CO2 environment of 5-10%. When phenol red is added, you can monitor pH changes from 6.2 (yellow) to 8.2 (red).

Each high-quality cell culture media by Capricorn Scientific is manufactured according to the originally published formula. We offer modifications essential to the consistent performance and stability of the culture medium. All our classical DMEM variants have been tested to optimally support the growth of your cells.