Overview

• Product nameAnti-CD31 antibodySee all CD31 primary antibodies ...
• Description
  Rabbit polyclonal to CD31
• Tested applicationsIHC-Fr,IHC-P,ICC/IF,IHC-FrFl,IHC-FoFr,WB more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide corresponding to C terminus of mouse CD31.

• Positive control
  • Jurkat and Raji cells. Tonsil.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, 10mM PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cardiovascular
  • Blood
  • Platelets

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Endothelial

  • Cardiovascular
  • Angiogenesis
  • Angiogenic Factors

  • Stem Cells
  • Mesenchymal Stem Cells
  • Surface Molecules

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Cell adhesion
  • Other

  • Cancer
  • Tumor immunology
  • CD markers

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Leukocyte recruitment
  • Cell adhesion molecules

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • T Lymphocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Monocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Neutrophil Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Myeloid
  • Thrombocytic Lineage

  • Cardiovascular
  • Cardiovascular Markers
  • Cell Markers
  • Endothelial Cells

Applications

Target

• FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
• Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
• Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
• DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
• Post-translational
  modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
• Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

• Target information above from: UniProt accession P16284The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 5175 Human
  • Entrez Gene: 18613 Mouse
  • Omim: 173445 Human
  • SwissProt: P16284 Human
  • SwissProt: Q08481 Mouse
  • Unigene: 376675 Human
  • Unigene: 514412 Human
  • Unigene: 343951 Mouse
• Alternative names
  • Adhesion molecule antibody
  • CD31 antibody
  • CD31 antigen antibody

  • CD31 EndoCAM antibody
  • Endocam antibody
  • FLJ34100 antibody
  • FLJ58394 antibody
  • GPIIA antibody
  • GPIIA' antibody
  • PECA1 antibody
  • PECA1_HUMAN antibody
  • Pecam 1 antibody
  • PECAM 1 CD31 EndoCAM antibody
  • PECAM antibody
  • PECAM-1 antibody
  • Pecam1 antibody
  • Platelet endothelial cell adhesion molecule antibody
  • Platelet/endothelial cell adhesion molecule 1 antibody
  • Platelet/endothelial cell adhesion molecule antibody

  see all

Anti-CD31 antibody images

• 

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - CD31 antibody (ab28364)This image is courtesy of an Abreview submitted by Ms Kelly Hudkins-Loya
  Anti-CD31 antibody (ab28364) at 1/50 dilution staining mouse tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Folowing heat mediated antigen retrieval the tissue was formaldehyde fixed and blocked with serum. The tissue was incubated with the primary antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary. The endothelium of vessels of any tissues are positive.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - anti-CD31 antibody (ab28364)

  ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Fromalin/ PFA fixed paraffin embedded tissue sections).

• 

  Immunohistochemistry (Frozen sections) - CD31 antibody (ab28364)
  ab28364 at a 1/300 dilution, staining human CD31 in fetal heart tissue by Immunohistochemistry, frozen tissue.

  See Abreview