Usage

This reagent is used to analyze cell cycle by flow cytometry. It is also used in conjunction with antibodies to study the expression of proteins during cell cycle. This reagent does not require dilution. Use 0.5 ml /test (1 x 10 6 cells) and incubate for 15 minutes at room temperature before analysis.

Contents

The reagent is suspended in a phosphate-buffered solution (pH 7.2) with 0.02% (w/v) sodium azide.

Preparation and Storage

Protect from light. Store at 4°C.

References

1. Douglas, R.S., A.D. Tarshis, C.H. Pletcher Jr., P.C. Nowell, and J.S. Moore. 1995. A simplified method for the coordinate examination of apoptosis and surface phenotype of murine lymphocytes. J. Immunol. Methods 188: 219-228.2. Kalejta, R.F., T. Shenk, and A.J. Beavis. 1997. Use of a membrane-localized green fluorescent protein allows simultaneous identification of transfected cell s and cell cycle analysis by flow cytometry. Cytometry 29:286-291.

Caution

Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoidaccumulation of potentially explosive deposits in plumbing.