Quality
Purity >90% by SDS-PAGE (contains entactin)
Tested for ability to initiate neurite outgrowth of NG-108 human neuroblastoma cells
Found negative for bacteria, fungi, and mycoplasma

Source
Engelbreth-Holm-Swarm (EHS) mouse tumor

Formulation
Frozen in 0.05 M Tris-HCl, 0.15 M NaCl, pH 7.4

Molecular Weight
900 kD (unreduced)

Preparation and Storage
Stable for at least three months at -70°C.

Handling
Recommended concentration: 1-10 µg/cm2 of growth surface depending on cell type