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蛋白酶K 来源于林伯氏白色念球菌 热销品！ 现货推荐！
详细描述
Proteinase K from Tritirachium albumVetec™ reagent grade, powder, ≥30 units/mg protein
Biochem/physiol Actions
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Application
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
法律信息
Vetec is a trademark of Sigma-Aldrich International GmbH
性质
grade Vetec™ reagent grade
form powder
mol wt mol wt 28.93 kDa
foreign activity Dnase ≤30 units/mg solid
storage temp. −20°C
