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Brilliant Violet 605™ anti-human CD34；Clone#：581Host/Isotype：Mouse IgG1, κ   

详细描述

Clone: 581
Isotype: Mouse IgG1, κ
Workshop Number: V MA27
Reactivity: Human, Cross-Reactivity: Cynomolgus
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 605™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Application Notes:

The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Application References:

1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
2. Felschow DM, et al. 2001. Blood 97:3768.
3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Human peripheral blood mononuclear cells

Human peripheral blood mononuclear cells were stained with CD45 FITC and CD34 Brilliant Violet 605™ (clone 581) or mouse IgG1, κ Brilliant Violet 605™ isotype control. Data was generated by gating on live CD14-negative cell population.



Description:

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
Other Names: Gp105-120, My10
Structure: 105-120 kD single chain mucin-like glycoprotein
Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
Function: Cell adhesion
Ligand Receptor: L-selectin, E-selectin
Antigen References:

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

  • 目录号：2317645
  • 品牌：Sony Biotechnology
  • 规格：25tests
  • 目录价：￥4183.50
  • 市场价格：￥2719.28
  • 会员价格：￥2593.77
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