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Biotin anti-human CD158e1 (KIR3DL1, NKB1)；Clone#：DX9Host/Isotype：Mouse IgG1, κ   

详细描述

Clone: DX9
Isotype: Mouse IgG1, κ
Reactivity: Human
Immunogen: Human NK cell clone VL186-1.6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
Application Notes:

The DX9 antibody reacts with the KIR (killer cell inhibitory receptor) designated NKB1 or KIR3DL1. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and restoring the NK cell cytotoxicity4,8.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Litwin V, et al. 1994. J. Exp. Med. 180:537. (IP)
2. Gumperz J, et al. 1996. J. Exp. Med. 183:1817.
3. Gardiner CM, et al. 2001. J. Immunol. 166:2992.
4. Bakker ABH, et al. 1998. J. Immunol. 160:5239.
5. Goodier M, et al. 2000. J. Immunol. 165:139.
6. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006. (FC)
7. Yawata M, et al. 2002. Immunogenetics 54:543.
8. Valiante NM, et al. 1997. Immunity 7:739.
9. Pascal V, et al. 2007. J. Immunol. 179:1625. (FC) PubMed
10. Lichterfeld M, et al. 2008. J. Exp. Med. 204:2813. (FC) PubMed
Human peripheral blood lymphocytes stained

Human peripheral blood lymphocytes stained with DX9 PE and CD16 FITC

Description:

CD158e1, also known as NKB1, is a 70 kD member of the immunoglobulin superfamily that is expressed on a subset of natural killer cells and T cells at varying levels among individuals. NKB1 is a type I membrane protein containing two immunoglobulin C2-type domains. The interaction of NKB1 with specific HLA-B antigens on a target cell (the HLA-Bw4 allele, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.
Other Names: NKAT3, NKB1, KIR-NKB1, p70
Structure: Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, 70 kD type I membrane protein
Distribution: Subset of NK and T cells
Function: Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-B antigens on target cell
Ligand Receptor: HLA-B antigens such as the HLA-Bw4 allele
Antigen References:

1. Colonna M, et al. 1995. Science 268:405.
2. D'Andrea A, et al. 1995. J. Immunol.. 155:2306.
3. Uhrburg M, et al. 1997. Immunity 7:753.
4. Gumperz JE, et al. 1996. J. Exp. Med. 183:1817.
5. Wagtmann N, et al. 1995. Immunity 3:801.

  • 目录号：2163520
  • 品牌：Sony Biotechnology
  • 规格：100μg
  • 目录价：￥3853.50
  • 市场价格：￥2504.78
  • 会员价格：￥2389.17
  • 数量：

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