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Alexa Fluor® 647 anti-human CD32；Clone#：FUN-2Host/Isotype：Mouse IgG2b, κ   

详细描述

Clone: FUN-2
Isotype: Mouse IgG2b, κ
Workshop Number: VI B051
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of acetone-fixed frozen tissue sections.
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Application References:

1. Kishimoto T, et al. 1997. Leucocyte Typing VI Garland Press. London.
2. Lerino F, et al. 1993. J. Immunol. 150:1794.
3. Personal communication.
4. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717.
5. Smeltz RB, 2007. J. Immunol. 178:4786.
6. Ackerman ME, et al. 2011. J Immunol Methods. 366:8. PubMed
7. Szittner Z, et al. 2013. PLoS One. 8:72401. PubMed
Human peripheral blood lymphocytes stained

Human peripheral blood lymphocytes stained with FUN-2 Alexa Fluor® 647

Description:

CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as FcγRII and FCRII. It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells. There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing. CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).
Other Names: FCR II, FcγRII
Structure: Ig superfamily, polymorphic transmembrane glycoprotein, 40 kD
Distribution: Monocytes, granulocytes, B cells, platelets
Function: Endocytosis, cytotoxicity, platelet aggregation, B cell function
Ligand Receptor: Aggregated IgG, immune complex
Antigen References:

1. Stuart S, et al. 1989. EMBO J. 8:3657.
2. Huang Y, et al. 1999. Scand. J. Immunol. 49:177.
3. Hisaka H, et al. 1999. Pathobiology 67:92.

This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  • 目录号：2116055
  • 品牌：Sony Biotechnology
  • 规格：25tests
  • 目录价：￥2752.50
  • 市场价格：￥1789.13
  • 会员价格：￥1706.55
  • 数量：

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