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PE anti-human CD88 (C5aR)；Clone#：S5/1Host/Isotype：Mouse IgG2a, κ   

详细描述

Clone: S5/1
Isotype: Mouse IgG2a, κ
Reactivity: Human
Immunogen: Recombinant peptide huC5aR N-terminal -NT (Asp15-Asp27)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Application Notes:

Clone S5/1 blocks the binding of C5a to CD88.
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Kiener HP, et al. 1998. Arthritis Rheum. 41:233.
2. Elsner J, et al. 1994. Blood 83:3324.
3. Oppermann M, et al. 1993. J. Immunol. 151:3785.
4. Li K, et al. 2012. Immunobiology. 217:65. PubMed
5. Kim SH, et al. 2013. Int Immunol. 25:623. PubMed
Human peripheral blood granulocytes stained

Human peripheral blood granulocytes stained with S5/1 PE

Description:

CD88 is a single chain protein with seven membrane-spanning regions and has a MW of 43 kD. CD88 is expressed by monocytes, neutrophils and eosinophils. Also, there have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release and superoxide anion production.
Other Names: C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
Structure: CD88 is a single chain protein with seven membrane-spanning regions and a MW of 43 kD.
Distribution: CD88 is expressed by monocytes, neutrophils and eosinophils. There has been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes and vascular endothelial cells.
Function: After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction: CD88 is coupled to heterotrimeric G proteins such as Gi. The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
Antigen References:

1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol Chem. 284:12363.
3. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
4. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
5. Buck E and Wells JA. et al. 2005. P. Natl. Acad. Sci. USA 102:2719.

  • 目录号：2321515
  • 品牌：Sony Biotechnology
  • 规格：25tests
  • 目录价：￥3165.00
  • 市场价格：￥2057.25
  • 会员价格：￥1962.30
  • 数量：

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