描述

Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4 (SEMA4A), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human SEMA4A (NP_071762.2) (Met 1-His 683) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its ability to bind biotinylated mouse SEMA4D in a functional ELISA.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

规格

Form:	Lyophilized
Purity:	≥ 90% by SDS-PAGE
Species:	Human
Expression:	Mammalian
Gene Symbol:	SEMA4A
Gene Aliases:	RP11-54H19.2, CORD10, FLJ12287, RP35, SEMAB, SEMB
Product Size:	50 µg
Protein Family:	Other Proteins
Endotoxin Level:	< 1 EU/µg
Protein Subtype:	Other Proteins
Molecular Weight:	73.4 kDa
Research Category:	Neurobiology, Development
Protein Tag or Fusion:	His Tag
Validated Application:	Assay Standard

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