详细描述

AlphaLISA no-wash immunoassay kit for detection of human TNFα in serum, buffered solution or cell culture medium. This kit contains enough reagents to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA technology allows the detection of molecules of interest in a no- wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.  

功能/优点

• No-wash steps, no separation steps
• ELISA alternative technology
• Sensitive detection
• Broad sample compatibility
• Results in less than 3 hours  

  详细信息

  Kit Content :

  • AlphaLISA anti-TNFα Acceptor beads
  • Streptavidin-coated Donor beads
  • Biotinylated anti-TNFα antibody
  • AlphaLISA human TNFα lyophilized analyte (0.1 µg)
  • AlphaLISA Immunoassay Buffer