详细描述

The AlphaLISA® Mouse/Rat Interleukin-17 (IL-17A) Detection Kit is designed for detection and quantitation of mouse or rat IL-17A in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

This kit contains enough reagents to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

AlphaLISA technology allows the detection of molecules of interest in a no- wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Interleukin 17A (IL17A) is the founding member of a group of cytokines called the IL17 family. The other members are IL17B, C, D, E, and F. Mature mouse or rat IL17A is a glycosylated homodimer formed of two 133 amino acid subunits. IL17A is produced by a subset of T helper cells. It is a proinflammatory cytokine that enhances T cell priming and stimulates macrophages, fibroblasts, endothelial and epithelial cells to produce mediators of inflammation like IL1, IL6, and TNFα. It is also critical for neutrophil activation and migration, and induces IL8, a key chemokine for neutrophils. IL17 has been involved in the proinflammatory patterns associated with joint inflammation and rheumatoid arthritis in mouse and human models and may be a novel therapeutic target for the treatment of these diseases.