详细描述

Lightning Blot Midi Transfer Stacks have everything you need for fast, efficient and reproducible electroblotting with most standard semi-dry blotters, including the Lightning Blotter from PerkinElmer. Each transfer stack consists of 3 vacuum sealed envelopes with pre-wet PVDF membrane, filter paper and transfer buffer. You can be ready to start the transfer in 2 minutes, and electroblotting is complete in 12 minutes or less. 0.2 micron membrane ensures complete transfer with minimal blow through for high and low molecular weight proteins.

Features/Benefits

• 2 minute set up for protein transfer from polyacrylamide gel
• Completer transfer in 6-12 minutes
• Improved reproducibility
• Quantitative protein transfer and retention for highest sensitivity

10 Midi Transfer Stacks configured to work with all standard 8 x 13 cm midi gels

Each transfer stack includes 3 vacuum sealed envelopes with 0.2 micron PVDF membrane, filter paper and buffer.

Transfer buffers

Envelope 1 (Anode 1): 200 mM Tris base + 10% (v/v) Methanol + filter paper

Envelope 2 (Anode 2): 25 mM Tris base + 10% (v/v) Methanol + PVDF membrane

Envelope 3 (Cathode): 25 mM Tris base + 40 mM Glycine + 10% (v/v) Methanol + filter paper