Loading
ATP LITE 300 ASSAY KIT 推荐升级产品一步法6016736
详细描述
ATPLite™ is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence assay is thealternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.The major advantages of this system are high sensitivity, excellent linearity, simplicity, fast results and the lack of cell harvesting or separation steps. Furthermore, the PerkinElmer ATPLite™ Constant Quanta™ assay system produces a long lived glow type signal with a half-life of greater than five hours, therefore a special luminometer with injectors is not required. The kit is ideal for use with the PerkinElmer TopCount® Microplate Scintillation and Luminescence Counter and the LumiCount® Microplate Reader in both 96- and 384-well microplates.
Advantages of ATPLite™
-
Long-lived luminescent signal
- half-life (t1/2) greater than 5 hours, depending on cell type and medium -
-
Rapid
- results generated in 15 - 25 minutes -
-
Simple and reproducible
- no separation steps
- only two reagent additions -
-
Homogeneous assay
- no cell harvesting or centrifugation required -
-
Sensitive
- down to 5 cells in 100µL medium (derived from CHO and HL-60 cells in 100µL medium) -
-
Wide linear dynamic range
- - ≥105 ( as derived from CHO and HL-60 cells)
Kit Content : 1 x 20 mL of mammalian cell lysis solution, 1 x 20 mL of substrate buffer solution, 3 x 10 mL vials of substrate (Luciferase/Luciferin) solution, (lyophilized), 1 vial of ATP standard (lyophilized), Instruction booklet.
Storage Temperature : +2-+8°C, DO NOT FREEZE!
