详细描述

The NADPH regenerating system consists of two reagents, Solutions A and B, which are sold separately. Solution A comprises NADP+ and Glc-6-PO4. When combined, Solutions A and B can be used for all NADPH requiring oxidase assays.

NADPH is a necessary cofactor in many xenobiotic metabolism reactions. NADPH is required for the measurement of oxidase activity catalyzed by P450s, FMOs, NADPH-P450 reductase, and many other oxidase enzymes. A common source of NADPH in an oxidase enzyme assay is an NADPH regenerating system that generates NADPH in situ using an enzymatic reaction. For example, glucose-6-phosphate dehydrogenase (G6PDH) will convert NADP+ to NADPH in the presence of the substrate glucose-6-phosphate (Glc-6-PO4).

The Corning NADPH regenerating system consists of two reagents, Solution A (NADP+ and Glc-6-PO4) and Solution B (G6PDH). Each reagent is sold separately. Combined, these two reagents form an NADPH regenerating system that can be used for all NADPH requiring oxidase assays (cDNA-expressed enzymes and liver fractions). At least 200 to 400 enzyme assays can be performed using one vial each of Solution A and B. The total number of assays that can be performed is dependent on a researcher‘s experimental design.