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PLATINUM PFX DNA POLYMERASE 250 REACTIONS #95514, #52806
详细描述
描述
Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic, hot-start method that improves PCR specificity (see figure). PCRx Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of Platinum® Pfx DNA Polymerase. The PCRx Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates. Platinum® Pfx DNA Polymerase provides:• 26 times higher fidelity than Taq DNA polymerase
• Amplification of fragments up to 12 kb
• Room temperature reaction assemblyApplications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum® Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.
Unit definition
One unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C.
规格
| Polymerase: | Platinum® Pfx DNA Polymerase |
|---|---|
| Hot Start: | Built-In Hot Start |
| Fidelity (vs. Taq): | 26 X |
| GC-Rich PCR Performance: | Medium |
| Reaction Format: | Separate Components |
| Product Overhang: | Blunt |
| Reaction Speed: | Standard |
| Final Product Size(s): | 12 kb or less |
| Product Size: | 250 reactions |
| Exonuclease Activity: | 3' - 5' |
| Shipping Condition: | Approved for shipment on Wet or Dry Ice |
内容及储存
Platinum® Pfx DNA Polymerase (250 units total, at 2.5U/µL) is a supplied with a vial (2 × 1 mL) of 10X PCRx Enhancer solution, a vial (2 × 1 mL) of 10X Pfx amplification buffer, and a vial (1 mL) of 50 mM MgSO4.
Designed for 250 reactions (at 1 unit each in a 50 µL reaction volume). Store at -20°C.
Designed for 250 reactions (at 1 unit each in a 50 µL reaction volume). Store at -20°C.
